Several efforts of photoanode treatment, such as surface modification, doping introduction, defect engineering, and heating treatment, have been developed to improve the photovoltaic performances of dye-sensitized solar cells (DSSC). Amid these options, alternative routes that require less demanding conditions are highly sought. Here we introduce an effective and convenient, non-vacuum low-temperature procedure to enhance the DSSC’s photovoltaic performances by photoanode pre-heating treatment prior to the dye immersion process. The experimental result showed that pre-heating treatment at 120 °C improves the efficiency and short-circuit current by about 28.4% and 19.1%, respectively. The conducted transient photovoltage measurements revealed that the increase of pre-heating temperatures leads to a longer electron lifetime and higher charge collection efficiency.